重慶市華雅干細(xì)胞技術(shù)有限公司 ，  ：

華雅集團(tuán)旗下授權(quán)代理TAKARA Cellartis干細(xì)胞產(chǎn)品

圖片為展示效果。實(shí)際是3套產(chǎn)品。

iPS向肝臟細(xì)胞定向分化試劑盒

產(chǎn)品英文名稱：Cellartis® iPS Cell to Hepatocyte Differentiation System

產(chǎn)品中文名稱：iPS向肝臟細(xì)胞定向分化試劑盒

產(chǎn)品介紹

以肝臟細(xì)胞為模型的實(shí)驗(yàn)越來越受到重視，在肝臟病變機(jī)理研究、藥物代謝研究、藥物毒理評估等方面有廣闊的應(yīng)用前景。然而，以個(gè)體原代肝細(xì)胞為材料來源會(huì)產(chǎn)生如取材困難、材料批間差大、無法長期穩(wěn)定供應(yīng)等一系列難以克服的問題。因此，通過iPS批量轉(zhuǎn)化生產(chǎn)肝細(xì)胞的方法就顯示出特別的優(yōu)勢。

Cellartis iPS Cell to Hepatocyte Differentiation System可以穩(wěn)定地、規(guī)模化地實(shí)現(xiàn)iPS向hepatocyte的誘導(dǎo)轉(zhuǎn)化生產(chǎn)，而且誘導(dǎo)轉(zhuǎn)化出的肝細(xì)胞能夠穩(wěn)定表達(dá)藥物代謝相關(guān)酶系統(tǒng)和藥物轉(zhuǎn)運(yùn)系統(tǒng)。

Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統(tǒng)，客戶既可以對某個(gè)特殊病人來源的iPS實(shí)現(xiàn)轉(zhuǎn)化，也可以利用cellartis供應(yīng)的iPS進(jìn)行標(biāo)準(zhǔn)化操作。使用本產(chǎn)品的過程起始于誘導(dǎo)人iPS轉(zhuǎn)化成為確定性內(nèi)胚層（Definitive Endoderm (DE)）細(xì)胞，然后誘導(dǎo)轉(zhuǎn)化DE至肝細(xì)胞。本產(chǎn)品是All-In-One型產(chǎn)品，實(shí)驗(yàn)所需的培養(yǎng)基、基質(zhì)（coating）等包括在內(nèi)。應(yīng)用本產(chǎn)品轉(zhuǎn)化約3 × 10⁶ hiPS cells,可以獲得約5 × 10⁶ 肝細(xì)胞（大約50 cm²面積的單層貼壁細(xì)胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System轉(zhuǎn)化得到的肝細(xì)胞除了清晰表現(xiàn)出肝細(xì)胞的*特征外（如肝細(xì)胞特異性標(biāo)志物、CYP系列酶等），還保留了原始細(xì)胞供體者的遺傳基因背景。另外，相比較從組織切除獲得的原代肝臟細(xì)胞（通常需要低溫保存），通過本產(chǎn)品系統(tǒng)轉(zhuǎn)化獲得的肝細(xì)胞在更長的時(shí)間跨度上保持著生理功能的穩(wěn)定性。這一點(diǎn)對于毒理學(xué)評估和病毒感染評估具有顯著的意義。

產(chǎn)品特點(diǎn):

高度可重復(fù)性強(qiáng)大的系統(tǒng) - *相同的方法已被證明可用于25種不同的iPS細(xì)胞系，因此無需針對您的細(xì)胞進(jìn)行優(yōu)化。

理想的藥物代謝和安全性研究 - 始終如一地生成具有不同遺傳背景的> 90％純的功能性hiPS細(xì)胞來源的肝細(xì)胞。

定制的起始材料 - 從任何疾病相關(guān)的iPS細(xì)胞系開始，創(chuàng)建準(zhǔn)確的肝臟疾病模型。

準(zhǔn)備好個(gè)性化醫(yī)療 - 為治療應(yīng)用制造針對患者的疾病特異性細(xì)胞。

擴(kuò)展實(shí)驗(yàn)窗口 - 肝細(xì)胞可用于功能測試至少11天。

Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.

Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.

Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.

Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

產(chǎn)品應(yīng)用:

iPS向肝臟細(xì)胞定向分化

組份：iPS細(xì)胞培養(yǎng)基+iPS分化內(nèi)胚層培養(yǎng)基+內(nèi)胚層分化肝細(xì)胞培養(yǎng)基

每種培養(yǎng)基都是*培養(yǎng)基

Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)：

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020)

? 100 ml DEF-CS 100 Basal Medium

? 800 µl DEF-CS COAT-1 (for 100 ml)

? 300 µl DEF-CS GF-1 (for 100 ml)

? 100 µl DEF-CS GF-2 (for 100 ml)

? 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030)

? 1 tube Definitive Endoderm Differentiation Coating (10 ml)

? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)

? 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

? 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

? 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

? 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

? 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

? 1 tube Hepatocyte Coating (7.5 ml)

自備試劑：

人多能干細(xì)胞

胎牛血清（FBS）或血清替代品（KO-SR）

PBS Dulbecco's（含有Ca2 +和Mg2 +的D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE選擇酶（1X），不含酚紅

保存：

iPS細(xì)胞培養(yǎng)基添加劑–20°C，基礎(chǔ)培養(yǎng)基和包被劑2–8°C

iPS分化內(nèi)胚層培養(yǎng)基和內(nèi)胚層分化肝細(xì)胞培養(yǎng)基所有組份都保存在–20°C。

Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1   2–8°C；GF-1, GF-2, and GF-3  –20°C

Cellartis Definitive Endoderm Differentiation Kit (Y30030)：–20°C

Cellartis Hepatocyte Differentiation Kit (Y30050) ：–20°C

產(chǎn)品性能：

Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

參考文獻(xiàn)：

Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance online publication. doi: 10.1007/s12015-015-9621-9

訂購信息

?華雅集團(tuán)旗下授權(quán)代理TTAKARA Cellartis干細(xì)胞產(chǎn)品，Cellartis的多能細(xì)胞培養(yǎng)系統(tǒng)包括iPS誘導(dǎo)多能干細(xì)胞株、iPS誘導(dǎo)多能干細(xì)胞和ES胚胎干細(xì)胞培養(yǎng)基，iPS基因編輯用單細(xì)胞克隆培養(yǎng)基和多能細(xì)胞檢測用單克隆抗體。

重慶市華雅干細(xì)胞技術(shù)有限公司以“專注干細(xì)胞研究，提升干細(xì)胞品質(zhì)”為發(fā)展理念，專業(yè)從事干細(xì)胞基礎(chǔ)研究、干細(xì)胞再生醫(yī)學(xué)臨床應(yīng)用研究及干細(xì)胞技術(shù)服務(wù)，自主研發(fā)產(chǎn)品熱賣中：人間充質(zhì)干細(xì)胞無血清培養(yǎng)基 

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